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RNA/DNA Folding

Predict secondary structure and minimum free energy (Zuker algorithm).

How it Works

This tool uses the Zuker recursive algorithm to predict the optimal secondary structure of a nucleic acid sequence.

Key Concepts

  • Minimum Free Energy (MFE): The structure with the lowest stability energy (kcal/mol) is typically the most stable state in nature.
  • Dot-Bracket Notation: A compact way to represent structure.
    • . : Unpaired base
    • ( ) : Base pair (opening and closing)
  • Temperature: Folding stability is temperature-dependent. The default is 37°C (body temperature).