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Golden Gate Assembly

Simulate restriction enzyme cloning with multiple parts. Supports BsaI, BbsI, and BtgZI.

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How it Works

Golden Gate Assembly is a method to assemble multiple DNA parts into a single valid circular construct using Type IIS restriction enzymes.

Key Concepts

  • Type IIS Enzymes: Enzymes like BsaI cut outside their recognition site, creating custom "sticky ends" (overhangs).
  • Parts: DNA fragments that have compatible overhangs. When cut and ligated, they assemble in a specific order.
  • One-Pot Reaction: Digestion and ligation happen simultaneously. The tool simulates this process to find all valid circular outcomes.

How to Use

Golden Gate Simulation

Simulates the digestion and ligation process of Type IIS restriction enzymes (BsaI, BbsI, BtgZI).

  • Parts: Define multiple DNA parts. Can be linear or circular.
  • Enzyme: Select the enzyme used for the reaction.
  • Logic: The tool finds recognition sites, cuts, and attempts to assemble compatible overhangs.

Instructions

  1. Select Enzyme: Choose the enzyme your parts are designed for (e.g., BsaI).
  2. Add Parts: Input your DNA sequences. Mark them as Circular if they are plasmids.
  3. Simulate: Click run to see if your parts successfully assemble into a circular plasmid.